Biuret reagent will indicate the presence of

Answers

Answer 1
Biuret reagent will indicate the presence of protein in a given sample. It is also known as the Piotrowski's test. This reagent consists of copper (II) sulfate and sodium hydroxide. It detects peptide bonds by the reaction of the copper ions in an alkaline solution. The copper ions would form violet colored complexes when peptide is present in the solution. From this test, concentration can be calculated since the intensity of the color depends on the amount of peptide bonds and according to the Beer-Lambert law concentration and the absorption of light is proportional. The concentration is calculated by a spectrophotometric technique at a wavelength of 540 nm. 
Answer 2

The Biuret test is a simple and quick method to detect proteins in a sample, and a positive result is indicated by a color change from blue to violet or purple. The correct answer is proteins.

Biuret reagent is used to test for the presence of proteins in a sample. The Biuret test is a chemical test used to detect the presence of peptide bonds in proteins and peptides. When Biuret reagent, which contains copper(II) ions, is added to a solution containing proteins, the copper(II) ions react with the nitrogen and oxygen atoms in the peptide bonds to form a violet or purple-colored complex. This color change indicates the presence of proteins.

The Biuret reaction is not specific to proteins; it can also react with compounds that contain two or more peptide bonds. However, it is most commonly used to detect proteins in biological samples. The test is not quantitative, but it can give a rough estimate of the amount of protein present based on the intensity of the color change.

 The Biuret reagent typically consists of a solution of potassium hydroxide (KOH) and copper(II) sulfate (CuSOâ‚„). The alkaline solution (KOH) denatures the proteins, exposing the peptide bonds, and the copper(II) ions form the characteristic colored complex with the peptide bonds.

 In summary, the Biuret test is a simple and quick method to detect proteins in a sample, and a positive result is indicated by a color change from blue to violet or purple.


Related Questions

Rank the homologous series for a set of alkanes by their boiling point? The boiling points for a set of compounds in a homologous series can be qualitatively predicted using intermolecular force strengths. Using their condensed structural formulas, rank the homologous series for a set of alkanes by their boiling point.
Rank these alkanes from highest to lowest boiling points
1....; The correct answer is heptane 3,3-dimethylpentane hexane butane. Thanks though, your tip helped!

Answers

The boiling point of a compound depends on the intermolecular forces binding them. For ionic and covalent compounds, they have higher boiling points compared to compounds joined by London forces. For alkanes, they would have the same intermolecular forces. It would then matter on the length of the carbon chain. The longer the length is, the higher is its boiling point. So, from the given choices, the highest boiling point would either be heptane or 3,3-dimethylpentane. Both have 7 carbon in its chain, but 3,3-dimethylpentane is branched so it has a lower surface area so it can easily be detached. That's why heptane is placed higher over 3,3-dimethylpentane . This is followed by hexane (6 carbons) and lastly the butane (4 carbons).

Alkanes from highest to lowest boiling points:

1. heptane2. 3.3 dimethyl pentane3. hexane4. butaneFurther explanation

Alkanes are saturated hydrocarbons with all carbon bonds being single bonds

General formula:

[tex]\large{\boxed {\bold {C_nH_{2n + 2}}}[/tex]

There are several rules for naming alkanes from IUPAC:

the name of the branch followed by the name of the main chain the main chain is the longest chain that has the most branches branches are given the name alkyl with the symbol -R, and begin with a number, with the position of the branch getting the smallest number more than one branch is prefixed in, tri, tetra, penta, etc. and in alphabetical order with the smallest number.

In determining the alkane boiling point, if in the same homologous series, then just look for alkanes with the largest relative molecular mass that will have a large boiling point too. So in an alkane in the form of a straight-chain, the more carbon atoms, the longer the main chain, the bigger the boiling point.

But if the relative molecular mass is the same, the alkanes have fewer branches will have a greater boiling point.

The more branches, the lower the boiling point, even though the number of carbon atoms is the same (as in isomers)

In isomers that have the same molecular formula and relative molecular mass, alkanes that have the fewest branches will have the largest boiling point.

So that the general determination of the alkane boiling point is (based on its priority)

1. relative molecular mass

the greater the higher the boiling point

2. main chain branch

the fewer branches the higher the boiling point

The branching on the main chain in the alkane structure causes the attraction between the molecules to be lower so that to release this attractive force only requires low energy, ie at low temperatures

The relative molecular mass of each alkane in the above problem is:

1. heptane: 1002. 3.3 dimethyl pentane: 1003. hexane: 864. Butane: 58

The relative molecular mass values ​​above indicate that there are similarities in the relative molecular mass values ​​of heptane and 3.3 dimethyl pentane. But because the unbranched chain has a higher boiling point so heptane has a greater boiling point than 3.3 dimethyl pentane

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the name of this hydrocarbon

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Keywords: alkanes, main chains, branches, boiling points, relative molecular mass

What is the molecular mass of cholesterol if .00105mol wieghs .406g?

Answers

Based on the information given in the question, the cholesterol has:
     a mass of 0.406 g
                 &
       moles of 0.00105 mol

Now, Molecular Mass = Mass  ÷  Moles
                                   
                                     = 0.406g  ÷  0.00105mol

                                     = 386.6667 g/mol

                                      ≈ 386.67 g/mol


Earth is the only planet that has a _____ and a _____.

Answers

if there are no selections than i would say a thick atmosphere and an unusual large moon.
Biosphere and hydrosphere

What is ionization energy?

Answers

The ionization energy is qualitatively defined as the amount of energy required to remove the most loosely bound electron, the valence electron, it is a very important substance. 

The ionization energy is the amount of energy needed to remove a valence electron. Valence electrons are the outermost electrons.

Assume you had a silver sphere with a mass of 1.50kg. calculate the diameter of the sphere (in cm). the density of silver is 10.5 g/cm^3. the formula for the volume of a sphere is... v = 4/3 3.14 r^3

Answers

First, we calculate the volume of the sphere through the following rule:
density = mass / volume
volume = mass / density = 1.5 / 10500 = 1.43 x 10^-4 m^3

Then, we use the rule of the volume to calculate the radius:
 v = 4/3 3.14 r^3
1.43 x 10^-4 =  4/3 3.14 r^3
r^3 = 3.4 x 10^-5
r = 0.0324 m

The last step is to get the diameter as follows:
diameter = 2 x radius = 2 x 0.0324 = 0.0648 m = 6.48 cm

Consider three 10-g samples of water: one as ice, one as liquid, and one as vapor. how do the volumes of these three samples compare with one another?

Answers

Given a 10 g sample of water in the form of ice, liquid, gas, we can observe how these phases behave. The volume for each of these samples would greatly differ given they have the same masses. For the solid, it will have a definite structure and would have smallest volume of the three phases since it is the most condensed structure of water. For liquid, it would have a volume that is larger than that of a solid but smaller than a gas. It is the intermediate phase between a gas and a solid. For the gas, it would be the one to occupy the largest volume since the molecules can freely travel and move around.
Final answer:

Ice has the largest volume, followed by liquid water, and then water vapor.

Explanation:

Water is one of the few substances on Earth that exists naturally in all three states: solid (ice), liquid (water), and gas (water vapor). The density of each state of water varies, with the vapor state being the least dense and the liquid state being the most dense. The solid state, which is ice, is less dense than the liquid state. So, in terms of volume, the ice sample will have the largest volume, followed by the liquid water sample, and then the water vapor sample, which will have the smallest volume.

Acids generally have an excess of ______ ions in solution, while bases usually have an excess of ______ ions in solution.

Answers

Acids generally have an excess of hydroxonium ions (H₃O⁺) in solution, while bases usually have an excess of hydroxide ions (OH⁻) in solution.

Write balanced chemical equation for the oxidation of fe2+(aq) by s2o2−6 (aq).

Answers

To write a balanced chemical equation for the given redox reaction, we need to know the half reactions of the substances involved. Redox reaction means oxidation-reduction reaction. Oxidation is when one loses electrons making it more positive while reduction is gaining electrons which makes it more negative. Iron here is being oxidized and will have the half reaction:

Fe2+(aq) ---> Fe3+(aq) + e+

 S2O2−6 is being reduced in the process and will have a half reaction of:

S2O2-6 + 4H+ + 2e- ----> 2H2SO3

Adding the two half reactions would yield to the balanced chemical equation. We do as follows:


2 (Fe2+ ---> Fe3+ + e+)
S2O2-6 + 4H+ + 2e- ----> 2H2SO3
----------------------------------------------------------------
2Fe2+ + S2O2-6 + 4H+  ----> 2Fe3+ + 2H2SO3  ( balanced oxidation - reduction reaction )

A combination reaction occurs when one reactant forms two or more products true or false

Answers

A combination reaction is a type of reaction which has two reactants that yields one product. For example, the electrolytic synthesis of water would be:

H2 + 1/2O2 ---> H2O

The reactions, hydrogen and oxygen, yield a water molecule. Therefore, the statement above is False. That type of reaction is a decomposition reaction, which is the opposite of combination.

Answer:

its true for apex ;)

Explanation:

Petrolyn motor oil is a combination of natural oil and synthetic oil. it contains 8 liters of natural oil for every 5 liters of synthetic oil. in order to make 728 liters of petrolyn oil, how many liters of synthetic oil are needed?

Answers

Petrolyn motor oil is a combination of natural oil and synthetic oil.
it contains 8 liters of natural oil for every 5 liters of synthetic oil.
natural oil / synthetic oil
8 / 5
let x= liters of synthetic oil and
728-x = liters of natural oil
8/5 = 728-x/x ,  8x = 5 (728-x)
8x = 3640-5x
8x+5x = 3640
13x = 3640
x = 280 litres
280 liters of synthetic oil is needed to make 728 liters of petrolyn oil.

If liquid a is more volatile than liquid b, which is also true? hints if liquid a is more volatile than liquid b, which is also true? liquid a has a lower vapor pressure than liquid

b. liquid a has stronger intermolecular forces than liquid

b. liquid a evaporates more slowly than liquid

b. liquid a has a lower boiling point than liquid

b.

Answers

liquid A has a lower boiling point than liquid B

Answer:

Liquid a has a lower boiling point than liquid  b.

Explanation:

Hello,

In this case, volatility is associated with three factors:

1. Vapor pressure: this is the pressure exerted by the gas molecules in equilibrium with the liquid. The higher the vapor pressure, the higher the volatility.

2. Intermolecular forces: are the interactions attracting or repelling the material's molecules. The stronger the forces, the lower the volatility as the molecules remain strongly closer.

3. Boiling point: is the condition of both temperature and pressure at which a liquid passes from liquid to gas. The lower the boiling point, the higher the volatility as more molecules easily pass from liquid to gas.

In such a way since liquid a is more volatile than liquid b, the answer is liquid a has a lower boiling point than liquid  b based on the aforesaid factors.

Best regards.

A student arranged the four cards sequentially in a row on the basis of the periods when these events occurred. The card showing the earliest event was placed on the extreme left. Which of these cards was placed third in the row?

Answers

If a student arranged the four cards sequentially in a row on the basis of the periods when specific events occurred specially in the early eras of civilization and then the card showing the earliest event was placed on the extreme left. The card that was placed third in the row is the picture showing a card label "First flowering plants appear on land'

The order of the pictures are:
1. Picture showing a card with label 'First life form appears in the sea'.
2. Picture showing a card with label 'First rocks formed on Earth'.
3. Picture showing a card with label 'First flowering plants appear on land'.
4. Picture showing a card with label 'First animal appears on land'. 

The sequences of the geological happenings in the periodical order is called the geological time's scale. The card of the third row depicts the first flowering plant.

What is the order of the events?

Events of the evolution can be explained based on the geological timelines and are arranged on the basis of their discoveries. In the question, the order of the events is arranged from the earliest to the newest.

The earliest event was the appearance of life in the sea followed by the rock formation. The third event was the appearance of the flowering plant on the land followed by land animals.

Therefore, the third card depicts the flowering land plant.

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which of the following is the most reasonable explanation for a drop in temperature of gas?

A) a decrease in the volume of the container
B) a decrease in the average kinetic energy of the gas particles
C)an increase in the average potential energy of the gas particles
D) an increase in gas pressure

Answers

B) a decrease in the average kinetic energy of the gas particles 

Answer: The correct answer is option B.

Explanation:

Average kinetic energy of the gas molecule is given by relation:

[tex]E_K=\frac{3}{2}\frac{R}{N_A}\times T[/tex]

[tex]N_A[/tex] = Avogadro Number

T = Temperature of the gas in Kelvins.

R = Universal gas constant

[tex]E_K\propto T[/tex]

With increase in temperature the kinetic energy of the gas molecule increases and vice-versa.

So, according to the question the explanation for a drop in temperature of the gas is decrease in average kinetic energy of the gas molecules.

Hence, the correct answer is option B.

What is present in the glucose molecule that is missing in the carbon dioxide molecule?

Answers

hydrogen .carbon dioxide is CO2 and glucose is C6H12O6

When copper sulfate reacts with sodium hydroxide solution, a precipitate of copper hydroxide and a solution sulfate are formed

Answers

In the equation above, Sodium Hydroxide is Na₂SO₄.

If you ionize this species, it results in Na⁺ + SO₄²⁻

∴ sulphate species is SO₄²⁻ (OPTION D)

Calculate the amount of heat required to vaporize 84.8 g of water at its boiling point.

Answers

You need to use the specifc latent heat of vaporization of water which you can find in internet.

I found 2,264.76 KJ / kg = 2,264.76 J/g

Now use total heat = specific latent heat * mass

=> 84.8 g * 2,264.76 J/ g = 192,051.65 J.

Answer: 192,051.65 J

Answer: Amount of heat required to vaporize 84.8 g of water is 191.7kJ.

Explanation: We are given 84.8 grams of water, to convert it into moles, we use the formula:

[tex]Moles=\frac{\text{Given mass}}{\text{Molar mass}}[/tex]

Molar mass of water = 18 g/mol

[tex]Moles=\frac{84.8g}{18g/mol}=4.71moles[/tex]

As we know that [tex]\Delta H_{vap}[/tex] for 1 mole of water at 100° C is 40.7 kJ/mol

So, to calculate the amount of heat required, we use the formula:

[tex]q=m\Delta H_{vap}[/tex]

Putting the values in above equation, we get

[tex]q=4.71mol\times 40.7kJ/mol[/tex]

q = 191.7kJ

if you drop a 50 gram piece of metal with a temperature of 125° Celsius into 1000 grams of water at 20° Celsius, what best describes what would occur?

Answers

As soon as the metal gets into the water a temperature transfer will start between both of them resulting in  

1. Metal will cool down. 
2. Some water will get evaporated.
3. Temperature of water will rise.
4. As the metal gets into the water, a sound like chissssss will be heard.

Hope it helps.
Don't be shy and say thx.

What is the concentration, in grams per liter, of a solution prepared by dissolving 0.00040 mol HCl in 2.2 L H2O? Assume that the volume of the solution does not change when the HCl is added.

Answers

On Ed. the exact answer is 0.0066.

Which of the following quantum number combinations is not allowed in a ground-state atom? (7, 4, -2, -½) (3, 0, 0, +½) (2, 1, 1, -½) (3, 2, 3, +½)

Answers

The last combination (3,2,3,+1/2) is not allowed.

The quantum numbers are (n, l, ml, s)

l, the second quantum number, may take values from 0 to n - 1; so:

n = 3 => l may be 0, 1, or 2 => the first and second quantum numbers are a valid combination.

The third quantum number, ml goes from - l to +l, so, given that l is lower than 3, ml cannot be 3. That is why this is not a valid combination.

An aqueous solution contains 0.050 m of methylamine, with kb = 4.4 x 10-4. what is the percent ionization and the ph of the solution?

Answers

Final answer:

To calculate the hydroxide ion concentration and the percent ionization of a 0.0325-M solution of ammonia, use the Kb expression and an ice table. The hydroxide ion concentration is 7.6 x 10-4 M and the percent ionization is 2.34%.

Explanation:

To calculate the hydroxide ion concentration and the percent ionization of a 0.0325-M solution of ammonia, we can use the formula for Kb. Kb is the equilibrium constant for the reaction of NH3 with water to form NH4+ and OH-. The equation is: NH3 + H2O ⇌ NH4+ + OH-. The Kb value for ammonia is given as 1.76 x 10-5.

First, we need to calculate the hydroxide ion concentration. We can use the Kb expression to set up an ice table and solve for the OH- concentration. Let x be the concentration of OH-. Since the initial concentration of NH3 is 0.0325 M, the initial concentration of OH- is 0 M.Using the Kb expression, we can set up the equation: Kb = (x)(x)/(0.0325 - x). Solving for x gives us x = 7.6 x 10-4 M. Therefore, the hydroxide ion concentration is 7.6 x 10-4 M.To calculate the percent ionization, we can use the formula: % ionization = (OH- concentration / initial ammonia concentration) x 100. Plugging in the values, we get % ionization = (7.6 x 10-4 / 0.0325) x 100 = 2.34%.

Which laws can be combined to form the ideal gas law?
Boyle’s law and Charles’s law
Gay-Lussac’s law and Avogadro’s law
Charles’s law, Avogadro’s law, and Boyle’s law
Dalton’s law, Gay-Lussac’s law, and Avogadro’s law

Answers

Charles’s law, Avogadro’s law, and Boyle’s law can be combined to form the ideal gas law.

What is Boyle's law?

Boyle's law is an experimental gas law which describes how the pressure of the gas decreases as the volume increases. It's statement can be stated as, the absolute pressure which is exerted by a given mass of an ideal gas is inversely proportional to its volume provided temperature and amount of gas remains unchanged.

Mathematically, it can be stated as,

P∝1/V or PV=K. The equation states that the product of of pressure and volume is constant for a given mass of gas and the equation holds true as long as temperature is maintained constant.

According to the equation the unknown pressure and volume of any one gas can be determined if two gases are to be considered.That is,

P₁V₁=P₂V₂

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What type of bond is easily disrupted in aqueous solutions?

Answers

Ionic bonds are easily disrupted in aqueous solutions, as they can ionize readily and as a result be surrounded by the solvent, water, where the partial positive and negative charges would be surrounding the appropriate charge of the ions.

A solution of sucrose in water has a boiling point that is _____ 100°C.

Answers

Depending on the quantity of dissolved surcose, surcose in water has a boiling point that ranges between 100 to 130 degree celcius.
It is essential to note that surcose does not boil, however it caramelizes when most of the water evaporates.

Based on this, the answer is "greater than"  since you'll have to heat above 100 for water to start evaporating.

Which drawing depicts the electron configuration of nitrogen?

Answers

Could I see the drawings? lol

At 25 °c only 0.0420 mol of the generic salt ab2 is soluble in 1.00 l of water. what is the ksp of the salt at 25 °c?

Answers

The Ksp, or solubility product constant, of a generic salt AB₂ at 25 °C with a solubility of 0.0420 mol/L is 2.96 × 10⁻⁴.

The student has asked to find out the Ksp of a generic salt AB₂ at 25 °C, when its solubility is given.

The solubility product constant (Ksp) is the equilibrium constant for the dissolution of a solid substance into an aqueous solution. Considering the stoichiometry of the generic salt AB₂, when it dissolves in water, it dissociates into one A²⁺ ion and two B⁻ ions.

The solubility equilibrium reaction is:
AB₂(s) → A²⁺(aq) + 2B⁻(aq)

Since the solubility is 0.0420 mol/L for AB₂, the concentration of A²⁺ will be 0.0420 M and the concentration of B⁻ will be 2 × (0.0420) M = 0.0840 M in the solution.

Using the expression for Ksp:

Ksp = [A²⁺][B⁻]² = (0.0420)×(0.0840)²

We calculate Ksp:

Ksp = (0.0420)×(0.007056) = 2.96 × 10⁻⁴

Thus the Ksp for AB₂ at 25 °C is 2.96 × 10⁻⁴.

According to Charles law, which two quantities are directly proportional?
A.) time and pressure
B.) volume and pressure
C.) volume and temperature
D.) pressure and temperature

Answers

d pressure and temperature hope this helps
C. Volume and temperature

During an effusion experiment, oxygen gas passed through a tiny hole 2.5 times faster than the same number of moles of another gas under the same conditions. What is the molar mass of the unknown gas? (Note: the molar mass of oxygen gas is 32.0 g/mol.)

Answers

Use Law of Graham.

rate A / rate B = √ (molar mass B / molar mass A)

rate A / rate B = 2.5

molar mass A = 32.0 g/mol

=> (molar mass B / molar mass A) = (rate A / rate B)^2

molar mass B = molar mass A * (rate A / rate B)^2

molar mass B = 32.0 g/mol * (2.5)^2 = 200. g/mol

Answer: 200 g/mol

Answer:

2.0 x 10^2g

Explanation:

If you reacted 88.9 g of ammonia with excess oxygen, what mass of nitric oxide would you expect to make? You will need to balance the equation first. NH3(g) + O2(g) -> NO(g) + H2O(g)

Answers

1) Balanced chemical equation

4NH3(g) + 5O2(g) ---> 4NO(g) + 6H2O(g)

2) State the molar ratios

4 mol NH3 : 5 mol O2 : 4 mol NO : 6 mol H2O

3) Convert 88.9 g of ammonia to moles, using the molar mass

molar mass of NH3 = 14 g/mol + 3 * 1 g/mol = 17 g/mol

number of moles = mass in grams / molar mass = 88.9 g / 17 g/mol = 5.23 mol NH3

4) Make the proportion

4 mol NO / 4 mol NH3 = x / 5.23 mol NH3=> x = 5.23 mol NO

5) Convert 5.23 mol NO to grams

molar mass NO = 14 g/mol + 16g/mol = 30 g/mol

mass = number of moles * molar mass = 5.23 mol * 30 g/mol = 156.9 g ≈ 157 g

Answer: 157 grams

Final answer:

The mass of nitric oxide expected to be produced from reacting 88.9 g of ammonia with excess oxygen is 156.7 g after balancing the chemical equation and performing stoichiometry calculations.

Explanation:

Calculating Mass of Nitric Oxide Produced from Ammonia:

To find the mass of nitric oxide that can be produced from reacting 88.9 g of ammonia with excess oxygen, first we need to balance the chemical equation. The balanced equation is:

4NH3(g) + 5O2(g) → 4NO(g) + 6H2O(g)

Next, we use the molar mass of ammonia (NH3) which is 17.03 g/mol to convert 88.9 g of NH3 to moles:

88.9 g NH3 ÷ 17.03 g/mol = 5.22 moles of NH3

Since the balanced equation shows that 4 moles of NH3 produce 4 moles of NO, we can calculate the moles of NO produced from 5.22 moles of NH3:

(5.22 moles NH3) × (4 moles NO / 4 moles NH3) = 5.22 moles NO

Finally, using the molar mass of NO (30.01 g/mol), we find the mass of NO produced:

(5.22 moles NO) × (30.01 g/mol) = 156.7 g NO

Therefore, we expect to make 156.7 g of nitric oxide from the reaction of 88.9 g of ammonia with excess oxygen.

How many moles of libr are contained in 347 g of water in a 0.175 m libr solution?

Answers

Molarity = number of moles of solute/Kg of solvent

Molarity is given in the problem as 0.175 m
Mass of solvent = 347 gm = 0.347 Kg

Therefore,
0.175 = number of moles of LiBr / 0.347
number of moles of LiBr = 0.175 x 0.347 = 0.060725 moles

Answer:

Molaridad = número de lunares de soluto/Kg de disolvente

La molaridad se da en el problema como 0,175 m

Masa de disolvente = 347 gm = 0,347 Kg

por lo tanto

0.175 = número de lunares de LiBr / 0.347

número de lunares de LiBr = 0,175 x 0,347 = 0,060725 lunares

Explanation:

A glucose solution is 3.25% glucose by mass and has a density of 1.03 g/ml. what mass of glucose is contained in 58.2 ml of this solution?

Answers

The density is the mass per unit volume. It is calculated from the formula d = m/V.
d = density
m = mass
V = volume.
We have here density = 1.03 g/ml, volume = 58.2 ml.
m = d * V = 1.03 * 58.2 = 59.946 grams.
Since that glucose is only 3.25% of the mass of the solution.
Therefore mass of glucose = 3.25/100 * 59.946 = 1.948 grams.
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