You run a PCR reaction for five cycles starting with a single DNA duplex. Theoretically, how many copies of your sequence would you now have?

8
24
12
16
32

Answers

Answer 1

Answer:

32

Explanation:

PCR is a technique that amplifies a small DNA sample. When a single molecule of a double-stranded DNA enters the first round of PCR, each of the two strands of the DNA serves as a template and results in the formation of two new DNA molecules. If the second round of PCR is performed and both molecules of DNA enter it, each of them would be amplified into two DNA molecules. So, there are 4 DNA molecules by the end of the second round of PCR. If five rounds of PCR are carried out starting with a single DNA molecule, a total of 2^5= 32 DNA molecules will be obtained at the end.

Answer 2

Final answer:

After five cycles of PCR reaction starting with a single DNA duplex, you would theoretically have 32 copies of the DNA sequence, as PCR doubles the number of copies with each cycle.

Explanation:

If you run a PCR reaction for five cycles starting with a single DNA duplex, each cycle will double the number of copies of your sequence. This is because the PCR process, which consists primarily of the steps of denaturation, annealing, and extension, doubles the amount of double-stranded target DNA with each cycle. So, starting with a single DNA duplex, after one cycle there would be 2 copies, after two cycles there would be 4 copies, and this doubling continues with each cycle. Following this pattern:

After the 1st cycle: 2 copies

After the 2nd cycle: 4 copies

After the 3rd cycle: 8 copies

After the 4th cycle: 16 copies

After the 5th cycle: 32 copies

Theoretically, you would have 32 copies of your sequence after five cycles of PCR.


Related Questions

Muscle cell activity increases and ATP decreases below the set-point, a sensor molecule detects low ATP and activates protein kinase A, protein kinase A activates protein kinase B, protein kinase B activates transporters that absorb glucose into the cell, glucose is metabolized. This is a _________.

Answers

Answer: Feedback mechanism

Explanation:

Feedback mechanism is the body's way of maintaining physiologic processes in the body. These physiologic processes include maintaining homeostasis, production of energy, hormones as well as enzymes required for chemical reactions.

There are 2 types of feedback mechanism:

Positive feedback: The stimulus (message) sent to the brain, produces prolonged increased production or increased activity in the body.Negative feedback: In negative feedback, the stimulus causes production, secretion or activity till a set point where the body knows it has taken enough. This causes a self termination of the process.

In the increased muscle activity, the increase in activity in turn causes a decrease in energy (ATP) and serves as the stimulus leading to the release of enzymes which in turn lead to increased absorption of glucose which will be used by the body to produce more ATP needed for the muscular activity. At the end of the muscular activity, the body recognizes that it doesn't need an increased absorption of glucose to support increased activity anymore and glucose absorption normalizes.

Billy Smart had been experiencing ophthalmalgia since completing a deck in his backyard. He asked his optician about it and she suggested Billy see an ophthalmologist. Ophthalmoscopy revealed a small cut on the eye's surface (the cornea), and antibiotics were prescribed. What is ophthalmoscopy?

Answers

Answer:

Ophthalmoscopy is also known by the name funduscopy is used to look into the different eye structure especially in the fundus region. This is involved in the normal physical examination.

During the examination, the complete information about vitreous humor, retins and discs can be understood by opthalmoscopy. The individual have any problem in the eye and its cure can be done by the ophthalmologist. In case of any eye emergency the individual must go for theopthalmoscopy.

With an apical four-chamber view, the left ventricle is generally the same size as the right ventricle. A. True B. False

Answers

Answer: The statement is false

Explanation:

The wall of the left ventricle is thicker and more muscular than that of the right ventricle, this is because it is through the left ventricle that blood is pumped out of the heart to all parts of the body.

Therefore, the left ventricle is NOT the same size as the right ventricle

The statement "With an apical four-chamber view, the left ventricle is generally the same size as the right ventricle" is

B. False.

In an apical four-chamber view of the heart, the left ventricle is generally not the same size as the right ventricle.

The left ventricle is larger and has thicker walls compared to the right ventricle. This is because the left ventricle needs to pump blood to the entire body through the systemic circuit, which requires more force, while the right ventricle pumps blood to the lungs through the pulmonary circuit, which is a shorter distance and requires less force.

Considering an X-linked dominant trait, if an unaffected woman and an affected man decide to have children, which of the following statements is TRUE about the possibilities for their children?
a. Their sons are expected to be heterozygous for the associated gene.
b.Their daughters are expected to be heterozygous for the associated gene.
c. All their children, whether male or female, are expected to show the dominant trait.
d. All of their sons are expected to show the dominant trait.
e. Their daughters are not expected to show the dominant trait.

Answers

Answer: B - Their daughters are expected to be heterozygous for the associated gene.

Explanation: In X - linked dominant trait,

1. Both males and females are affected; often more females than males are affected.

2. Affected fathers will pass the trait on to all their daughters.

3. Affected sons must have an affected mother.

4. Affected daughters must have either an affected mother or an affected father.

5. Affected mothers (if heterozygous) will pass the trait to ½ of their sons and ½ of their daughters.

Check the attached image to see the illustration of how an X - linked trait can be passed from an affected father and unaffected mother to their offspring.

PROTOZOAN CULTURES- Submit your observations.

Answers

Protozoans are single celled eukaryotes that exhibit plant and animal like features. Some of the examples of protozoans are - Euglena, Paramoecium and Amoeba. To observe these organisms under the microscope, a small amount of it from the culture jar is taken and observed at different magnification. Euglena moves with the help of a flagella and hence, is classified as flagellate. Paramoecium moves with the help of cilia and hence, classified into ciliate. Amoeba moves with the help of pseudopodia and thus, it is a sarcodine.  

All the three organism has nuclei. However, paramecium have two nuclei called as a micronucleus and macronucleus. Euglena lacks food and contractile vacuole. Euglena also has an organelle called as chloroplast which is found mostly in plants.  

Functional magnetic resonance imaging scans provide computer-generated images of brain structures and activities by aiming a powerful magnetic field at the body.

1. True
2. false:

Answers

Answer: True

Explanation:

The magnetic resonance imaging scans provide the picture of the body. This image is computed image of the body.

The MRI scanners use the strong magnetic field in order to obtain the image of the body and organs.

This image is used to detect the problems in the body which can be cured using certain medications if detected on time.

In the 1800s the most widely favored explanation of genetics was "blending." Explain the concept of blending, and then describe how Mendel’s "particulate" (gene) hypothesis was different.

Answers

I need the answer to!!!

Help!! I'm stuck on this....

Suppose a stream has a low volume but a steep gradient. How might the
stream change the land? Provide your reasoning.

Answers

Answer:

The steep gradient causes the erosion of soil.

Explanation:

Steep gradient is the slope of the land where the stream is present or any flow of runoffs.As the slope of stream flowing increases, the velocity of water also increases causing the erosion of soil.It causes the land to get deformed by the sweeping of soil making difficult for plants and grasses.The steep gradient runoffs negatively impacts the aquatic living thing.
Final answer:

A low-volume stream with a steep gradient can change the landscape through erosion and sediment transport, forming features such as gorges, ravines, and river terraces over time.

Explanation:

Even a stream with a low volume but a steep gradient can significantly change the land. The steep gradient means the stream flows rapidly, creating a powerful force that can erode soil and rocks, transporting them downstream. This process can form features such as gorges and ravines.

Over time, this constant erosion and sediment transport can remodel the landscape, deepening valleys and altering the course of the stream. On a smaller scale, it may also create river terraces, which are flat areas alongside the stream where sediments accumulate.

Learn more about Erosion and Sediment Transport here:

https://brainly.com/question/32312372

#SPJ3

Teleological moral theories are also known by the term a. nonconsequentialist. b. deontological. c. consequentialist. d. epistemological.

Answers

Answer:

C. consequentialist

Explanation:

Teleological Ethical Theories deals with the aftermath of actions I.e. the primary guidelines for our actions as either morally right or wrong relies on the good or evil results it produced.

The term Teleological is a Greek word telos, “end” and logos, “science”.

Consequentialism believes the results of one's actions are the ultimate reason for concluding on the rightness or wrongness of that action. A consequentialist views a morally right action on the good outcome or consequence that results from it.

Take for example, some view lying as wrong. But if telling a lie is necessary to redeem or save a person's life from certain situations, consequentialism holds the belief that it is the best thing to do.

Final answer:

Teleological moral theories focus on the consequences of actions and are also known as consequentialist theories.

Explanation:

Teleological moral theories are a group of ethical theories that regard the consequence or the purpose of actions as the basis for determining their moral value. These theories contrast with deontological theories, which argue that actions are right or wrong in themselves, irrespective of their consequences. The term "teleological" is derived from the Greek word "telos," which means "end" or "purpose." Therefore, the correct answer to the question of which term is also known for teleological moral theories is c. consequentialist. Bentham's Utilitarianism is an example of a consequentialist theory because it judges the morality of an action by its outcomes, specifically, by whether it maximizes pleasure or happiness.

Following knee replacement surgery 10 days earlier, an older adult client diagnosed with an infection in the knee has a synovial fluid culture ordered. Obtaining the sample helps to determine the causative microorganism and to select an appropriate antibiotic. The above course of events characterizes what major belief system? A. Holistic paradigm B. Scientific paradigm C. Analytic paradigm D. Magico-religious paradigm

Answers

Answer:

The correct option is B) Scientific paradigm

Explanation:

The scientific paradigm focuses on the infection and the microorganism for the occurrence of the infection. A scientific paradigm also refers to the treatment of these infections through pharmaceutical or surgical approaches. Hence, option B is correct.

Other options, like option A, is not correct because a holistic paradigm focuses on the connection between the mind, body and the soul. Option C is false because the analytical paradigm is not a part of the major health belief system.

Other Questions
Which of the following definitions best describes the term devolution?A. the creation of a united central governmentB. the establishment of a coalition between member statesthe breakup of a state into smaller, independent statesD. the transfer of power from one dictator to another A researcher argues that individuals with low self-control are more likely to commit crimes than individuals with high self-control. The independent variable in this proposition can be stated most clearly as:____________ k. Starbucks reports Income from Equity Investees in its income Statement. Using the narrative information provided in this case, describe the nature of this type of income. That part of the discipline of anthropology that debates issues of human nature that relate directly to the decisions of daily life and making a living is called________. Cameron is having a party and needs to write step-by-step directions on the invitation. Which type of list would yourecommend he use for the invitation?a Directional listb. Numbered listC Bulleted listd Outline listPlease select the best answer from the choices provided Which diagram best explains a cause and effect relationship that has shapedU.S. Politics?hascontributedtoMajority rule inCongressThe existence ofmany influentialpolitical partieshavecontributedtoB.Long-establishedparty loyaltiesThe persistenceof the two-partysystemhavecontributedtoOc.) CElections thatreward candidateswho finish secondor thirdThe persistenceof the two-partysystemhavecontributedRestrictions onwho can receivepublic campaignfundsThe existence ofmany influentialpolitical parties 1 Round the number 5.014937 to the nearest hundredth. Technician A says clutch slippage can best be diagnosed with the vehicle stopped, parking brake applied, placing the transmission in 4th gear, and slowly releasing the clutch pedal to see if it stalls the engine. Technician B says an excessively slipping clutch can cause friction surface warpage or hot spots. Who is correct? Select one: a. Technician A b. Technician B c. Both Technician A and Technician B d. Neither Technician A nor Technician B 5.How can time out become a positive experience for children? The Ortega family made 15/16 pound of confetti for the Annual fiesta celebration in San Antonio they use 1/4 pound to make field eggs how much confetti is left next year Password is an example of an authentication mechanisms that is based on " what an entity has".True or false? A lithium atom has lost an electron comes near a chlorine atom that has gained an electron. what happens? A man is parasailing above a lake at an angle of elevation of 32 from a boat. If 129.5 meters of line connects the boat to the parasail, how many meters above the lake is the man to the nearest tenth of a meter? A)68.6 m B)80.9 m C)109.8 m D)152.7 m What is the criminal penalty for engaging in the real estate brokerage business in Texas without firstbecoming licensed by TREC? A train travels 55 km south along a straight track in 34 minutes. What is the train's average velocity in kilometers per hour? Highlight one example of figurative language and one example of language that has positive connotations. Identify your examples and explain how they help the reader better understand the conditions the factory workers endured at that time The diagram shows the structural formula of benzene. The empirical and the molecular formulas of benzene are, respectively -fCH, C2H2gCH, C3H3hC3H3, C6H6jCH, C6H6 Five firms are currently producing and selling in a market. When two more firms enter the market, economists expect that the equilibrium price will ________ and the equilibrium quantity will ________. James Madisons response to Patrick Henrys arguments was to warn that _____.without ratification of the Constitution, the Union could break upforeign powers were waiting to pounce on the individual states unless they became strongerPatrick Henry preferred no government at all, a dangerous condition in which no one would be safethe delegates were too exhausted to start over again to rewrite the Articles of Confederation(please only help if your sure thanks) Which of the following choices best illustrates the concept of Adam Smith's "invisible hand"? A Vietnamese farmer grows rice, an exporter ships it to the United States, and a grocer in New York sells it to a customer. A fishery's stock of fish becomes depleted due to overfishing as boats from around the world converge. A government regulates a firm to clean up the pollutants the firm has released as part of its production process. An apartment building that provides much-needed housing is built on the site of the only playground for children in that town. Steam Workshop Downloader